Waters BioSuite RPC column offerings include a C18 (pC18) and a phenyl (pPhenyl) chemistry bonded to a pH stable, methacrylic ester-based polymeric resin. The 500 angstrom pore size of the pC18 base matrix accommodates proteins up to 2,500,000 Daltons while the 1,000 angstrom pore size of the pPhenyl base matrix accommodates proteins up to 5,000,000 Daltons. The BioSuite pC18, 2.5 µm, NP column contains a nonporous chemistry that yields superior chromatographic resolution compared to chromatography performed on the porous, pC18, 500, 7 µm RPC selection. However, greater protein or peptide binding capacity is possible using the porous, pC18, 500, 7 µm RPC column. The pC18 and pPhenyl RPC chemistries are available in 21.5 x 150 mm columns for “lab scale” isolations while a 2.0 mm x 75 mm column is well suited for narrow-bore HPLC and /or LC/MS applications.